You don't have to use the same port, but Windows is stupid enough to have to reinstall the drivers every time you use a new one.
If you are using a hub, ALWAYS plug the hub into the same port and ALWAYS plug the camera into the same hub port. You'll save yourself a heap of pain.
If you have USB3 ports anything before Win8 can be a nuisance. They don't enumerate USB3 ports the same way as they do USB2 and some drivers can't cope.
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