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Dunk, on Bias, this is the basic story:
You should ALWAYS use Bias to remove the read signal from your Flats (which are then the denominator in the calibration equation).
If you use Darks, then you don't need the Bias subtraction sub-model (since the Dark contains both the dark current and the bias signal, both of which are then subtracted in unison from your Lights). Unless you are using a bad pixel map instead of a Dark, in which case the Bias is needed here too.
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