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Hello Alistair,
Sorry to hear you are having an issue. As has been suggested check the power rails to see if the PIC is getting juice. The design does have reverse polarity protection but not short circuit protection. A DB9 connector generally ensures against pin shorting.
If you do have power the only other thing that can halt the PIC (assuming it is not damaged) is a problem with the crystal or associated capacitors. Power the board on while depressing the manual control button - this will put the PIC into boot mode and the manual control LED should rapid flash. If this does not happen that suggests the PIC has no power or the clock is not running. If the LED does rapid flash the PIC is alive and well but for some reason the application code has become corrupted. This is not likely but is possible so you could download the .HEX file from my website and reflash the PIC.
Report back and let us know how you get on.
Cheers,
Dave
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