I'll add to the chorus of not using in camera noise reduction.
I build a library of darks for each ISO/duration combination. They're named with the temperature details, so they're easy to match with the lights (also have temperature details in the file name).
Liveview is off (it's power hungy and generates a huge amount of heat), focusing, etc is all done via the laptop (BackyardEOS mostly, sometimes APT). Images are stored on the laptop, not the camera.
I've got a power adapter for the DSLR that I run through my break out box (so it works for 240/12V) - this also means I'm not limited by battery time and can go all night.
I almost always use PHD dithering when taking images, so there's a very slight position difference - this improves the result in DSS (which, btw, you should be using the latest beta if you aren't already).
Flats/Dark Flats are taken each session (about 10 each). For the backyard I have a convenient house floodflight near by I can switch on that gives a nice glow to the white cloth over the scope. When I'm at a dark site, I either do the flats in the late afternoon or use the car lights.
