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I think most will want to take darks as at the end of an imaging session, as Jarrod has said. Otherwise you're wasting 50% of your imaging time where you should be catching valuable light frames while your object is high in the sky. You can take dark frames after it's dropped too low for decent definition or after it's set. Certainly you get better temperature matching of dark frames with in camera noise reduction, but at a considerable cost, and temperatures don't normally vary so significantly across a few hours of the night that the darks will be too inaccurate.
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