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Without isolation a scope mounted to a floor upstairs will be susceptible to vibrations and flexure from anywhere in the house, not just your self moving around up there. Visually not a problem amybe, even for lunar and planetary video but long exposure DSO it's a dubious proposition.
You will also find that your house moves slightly with the seasons, dry earth, wet ground and that will throw any alignment out and you can't adjust it as you will be standing by the mount when you do so it will be affected.
I don't know what a dome on a roofline will do for later resale value either. I have read of some upstairs\attic ob builds but they have always involved an isolated pier going down to ground level. If you can do that then it's workable, expensive, but workable.
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