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John,
nice image.
Regarding flats...I was of the knowledge that you take the flats with the same ISO your imaging using a long enough exposure to get around halfway on the histogram.....also I use a white pillow case over the scope....dont know about this grey scale...I'm sure their is other methods yours may be 1.
No such thing regarding Best ISO...it depends on your target....some are using as low as 200 but most between 400-800...if your only taking short 30 second exposures you will find the higher ISO is better catching more light but the longer you go the lower the ISO the better.
I am between 400-800 ISO atm.
Take all your images in raws including Flats, Darks, Bias frames...then you can combine them in IP and make a master file.
Hope this help John.
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