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I personaly would have the Mirror lock on.
The shutter can be very violent..this will affect imaging at long focal length as the smallest movement will ruin your image.
Sure start with it off but I never take an image with it off.....normaly a 5 second delay is sufficent for me....this gives the scope time to settle if the shutter cause's the slightest of movement....I am talking very minor movement here.
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