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In my experience once you reach the number of subs around 35-40 there is not much difference when you add another 10, or more, and process them in one batch as 50, however, if you do the same as two separate batches and then integrate the two images – the result is different.
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Without any rejection you should get the same result either way. With a rejection algorithm you will get different results but I would expect most rejection algorithms to perform better with more subs - they would have a more robust estimate of each pixel value to work with. Might be an interesting experiment to see if the actual results match theory...