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Hi Peter,
Please check the attachment - I have further stretched your frame to highlight the amp glow. Again, I have seen this in other cameras with Sony's sensors so do not worry. It might be because of their higher sensitivity so that the CCD is picking up faint signal from within the camera. It gets bigger with longer exposures, but still is not noticeable at all in the light frames.
As for dark calibration, it will add more noise but hopefully at the same time remove more significant camera-induced artefacts. I only use bias subtraction (ICX814) and make a master bias from 250 frames.
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