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The biggest thing I learned when I started with a DLSR (And older Canon) was that they will produce loads of hot pixels, a 60 second dark frame looked like a colourful starfield.
The best bet is to shoot heaps of darks in whatever configuration you are going to use to image with. So if you will take 60 second shots at ISO 400 with no break between, shoot at least 40 darks in the same way (And at a similar air temperature) to create a master dark in whatever software you use for stacking. Likewise for any bias frame, shoot a heap and combine them for a good master bias.
If you are using a guider, dither between subs as that helps the stacking software reject any hot or cold pixels or fixed noise not removed by a master dark frame.
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