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Pete i suggest you read through some of the Cloudy Nights forum threads, the the Beginner and Intermeadiate Imaging sub-forum, on the ASI1600, and the topic covers both the colour and mono versions.
Gain in the ASI1600 can be thought of like ISO is to DSLRs. Offset is like brightness.
There is a sweet spot for Gain at 75 where you get maximum pixel well depth. Unity Gain is a Gain setting of 139, and an Offset of 20, and represents a good starting point for people new to the camera.
I do not use Nebulosity so i can't comment on your pic, i prefer SGP.
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