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Not really enough info Todd. I assume that you stacked darks and maybe flats
with each different group, and enough of them. DSLRs have a sweet spot for
astrophotography, which is the point on an ISO graph where the two different types of noise intersect. With popular Canon DSLRs it is usually 800, 1000,
or 1600. For example the EOS 1100D is 1600 and the EOS 600D is 800.
The further you deviate in either direction from the sweet spot, the more noise you will get, so your choices will not get a really good result, especially
160.
If you are using in camera noise reduction, and hence no darks, it is fine to stack different sub lengths and ISOs simultaneously.
If using darks, flats, etc, you need to stack them separately.
raymo
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