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Hi Martin
Yes, you do need separate flats for each filter. Not best practice - required.
Each one lets a slightly different amount of light through and refracts the light slightly differently. You'll see this in your flat wizard - no two filters should have exactly the same exposure time (assuming you're using a dynamic flat with a static light panel).
Focus doesnt matter. Leave it as is after your imaging session.
People often go overboard with flats. You may not need to take flats after every single imaging session if you're imaging setup is reasonably static. With my refractor and some precautions, I sometimes only need to take flats every month or so.
You are correct that the IMX571 likes bias calibration - keep doing that with the mono version. No need to take flat darks on this sensor.
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