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If these are 5 minute subs there is not enough time for flexure to be an issue. Either there is something wrong with the guide setup, something wrong with your drive(s) or something loose on your scope. With my crappy Meade LX200GPS mount I often get trailing on the first image in a series. Sometime the scope just moves off (probably a small amount of lag in the worm). However, 2nd and subsequent images in that series are fine.
Cheers
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