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Can you try a long single exposure? That would accentuate any tracking issues, at that focal length you should be able to get a minute or more unguided without major issues but if you have a polar alignment issue it should show up reasonably clearly by then, and any mechanical issues would be a lot more apparent.
If you used the ASI294 I would suggest a minute, at 120 gain and -15 degrees (It is a 294MC Pro isn't it? I know you have mentioned it elsewhere before but cant think in which thread) The area covering NGC253 and NGC288 should fit on the sensor of the 294 through the RASA.
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