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maybe check that your bias has not set itself to something weird and also check that the cooler is actually getting the chip temperature down to the target. Nebulosity threw a wobbly when I ran the camera at low bias without cooling - it recognised that it was being presented with negative results and refused to work.
also, does the camera work in SharpCap with the supplied USB3 cable? At least you could establish that the camera still functions.
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