I don't have PI software, so can't try out their superbias process/algorithm (any idea how it works, exactly?). But I definitely only use bias subtraction in my ultra-short flats, since read noise will completely dominate over any (minimal) dark current noise that might arise in 0.1s.
Hot pixels effects should also be really minimal in 0.1s flat exposures, but one could remove them with a BPM and dithering if it was a concern.
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