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I’ve also experimented with stacking different sub lengths and ISO settings on my Canon 600D and found that it just doesn’t get a satisfactory result
My sweet spot is ISO 800 and my sub exposure times vary with each object I image at my 2 locations , Sydney Bortle 8 and Narrawallee south coast NSW Bortle 3
As Ryan said take plenty of subs, take plenty of darks ( ratio of at least 3:1 ) at same settings preferably straight after your subs and make sure your histogram curves are in the 1/3 zone towards the right side where possible ( obviously City and Suburban sky glow blows your histogram way out )
For exposures beyond 1 minute or so using a DSLR , dithering is a must do for fixed pattern noise reduction and dark current noise reduction
All the best
Good Luck !
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