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As far as I know it should be the sensor temperature as that is where the thermal noise is generated. If you are using one of the dslr astro programs (BackyardEOS or APT) then you can have the sensor temp inserted as part of the filename which is very handy. They also support a USB humidity/temp device called TemperHum which will give (obviously) temp and humidity and calculate the dewpoint for you.
Adam
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