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Second looks the best for sure. As for darks and flats subtration with a DSLR camera cooled; I used a darks and flats library. Using IP I just used the standard darks subtraction and this produced good results on many occasions. I don't agree with H that you need to take them on the night. I found variations of up to 5 degrees or so did not really matter much. Especially in wide field mode.
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