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When you use a quide scope even if it's bolted and has zero flexure you still can get flexure between the guidescope and the main imaging telescope. If it's a refractor then it's less likely but if it has a mirror and depending on its mounting, size and weight YMMV. Now the only way to get rid of flexure 100% is to guide through the same light path you're imaging.
If you use a guidescope on a celestron SCT you will likely get flexure because the primary will move. If you do the same on a big newt same thing. If you image with a scope that is very tight mechanically then you might get away with it.
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