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I am quite anal about darks and flats. If you want to get a signal that is very faint any erroneous 'calibration' will insert artefacts.
I always use up to date darks at the set temperature and duration. Thirty at least.
I do not bother with bias frames as the darks already have them embedded. Bias frames are only needed to fudge some sort of temperature or exposure calibration.
With flats I am even more anal as I take darks for the flats and calibrate them accordingly. I also expose at least thirty flats to get a statistically relevant average.
Bert
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