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A signifcant amount of noise accumulates in each dark frame the longer the camera is left on I've got a collection of darks where you can see this clearly especially as the ambient temperature rises.
No doubt that is why cripser clearer frames are captures in the winter months.
Ideally IMO turning the camera off between frames would be the go but as you know this isn't practical better is someway of cooling the camera which I am working on.
Cheers
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