Yes and no... I'd keep originals of the images as hi-res as possible (as file size/storage allows).
When printing make sure the dimensions you wish to print don't cause the resolution to fall to a point where image quality degrades i.e. 300dpi
Generally screen/online usage is the only time I change the res of an image (always using a duplicate) bringing it down to x-width pixels X x-height pixels at 72dpi. The aim being to reduce the files size for upload and delivery.
Of course I'm only speaking from a graphic design perspective. I'm a complete astronomy newbie and have zero astrophotography experience so someone with more experience there may be able to advise you better? The principals would be the same however I imagine.
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