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Its also good practice to subtract a bias from your flats when applying your flats.
I take about 16 bias images and then stack using median combine. Then when I apply flats to my image I check the box in CCDstack to subtract a bias and type in the file location for the bias.
Also a careful dark subtract helps with flats.
I use about 16 darks to form a master dark again median combined. They are the same duration and temperature as the light exposures I have taken. It makes sense to standardise these as much as possible so you don't have to make lots and lots of dark masters.
Pick a temperature you know your camera can achieve in winter and one for summer or one for all year round if you want to minimise the work.
Doing an accurate dark subtract may seem not relevant to getting a good flat applied but I can assure you it does. All you need is a scope that requires more accurate flats and you'll see how vital it is.
Greg.
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