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Chris, aim for about 10-15 steps in each direction for calibration. If it takes less, decrease the max step. If it takes more, increase it.
Somewhere in the 1500-2500 range will probably get it right, but I've never used a guide scope that short so you'll need to experiment.
You will do best with the guide scope slightly defocused. This reduces the effect of the guider trying to chase the seeing.
I know from experience that min motion works at 0.1 pixels with a 400mm guide scope. On a reasonable night, set min motion to something in the 0.1 to 0.5 pixels range and watch the graph. Tweek the value up and down to get the smoothest line.
It is not exactly a black art, but it isn't something you'll get right in a few minutes, and every combination of guide scope, guide camera, and mount behaves differently.
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