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I should add that some programs behave differently. Some programs will subtract master bias information from dark frames if you load a master bias to make a master dark. Astro Pixel Processor does not do that. The master darks do not have master bias information included even if you have a master bias loaded when you make the master dark. From there the light frame calibration depends on what you load. If you load a master bias but no master dark it will subtract the master bias from the light frames, if you load both a master bias and a master dark, it subtracts the master dark, but not the master bias as the master dark contains the bias information as well..
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